Selank (5 mg vial)

Protocol Overview

Concise summary of the regimen.

Dosing & Reconstitution

Frequency: Inject once daily subcutaneously. A common cycling pattern is 4 weeks on, 4 weeks off to prevent tachyphylaxis, though continuous daily use has been studied in clinical trials for up to 14 days with good tolerability. Some practitioners recommend administering 5 days per week with 2-day breaks.

Reconstitution Steps

1. Draw 3.0 mL bacteriostatic water with a sterile syringe.
2. Inject slowly down the vial wall; avoid foaming.
3. Gently swirl until dissolved (do not shake vigorously).
4. Label and refrigerate at 2 to 8 °C, protected from light. At 1.67 mg/mL, 1 unit = 0.01 mL » 16.7 mcg on a U-100 insulin syringe.

Storage Instructions

Proper storage preserves peptide quality.

• Lyophilized: store at -20 °C for long-term stability; refrigeration at 2 to 8 °C acceptable for shorter

periods.

• Reconstituted: refrigerate at 2 to 8 °C; stable for approximately 1 month with minimal potency loss; avoid

freeze-thaw.

• Allow vials to reach room temperature before opening to reduce condensation and moisture uptake.

Supplies Needed

Plan based on an 8 to 16 week protocol with 4-week cycles and gradual titration.

Peptide Vials (Selank, 5 mg each):

• 8 weeks (including one 4-week cycle): ~5 vials.
• 12 weeks (one full cycle + partial second): ~7 vials.
• 16 weeks (two complete cycles): ~10 vials.

Insulin Syringes (U-100):

• Per week: 7 syringes (1/day).
• 8 weeks: 56. 12 weeks: 84. 16 weeks: 112.

Bacteriostatic Water (10 mL bottles):

• 8 weeks (5 vials): 2 bottles. 12 weeks (7 vials): 3 bottles. 16 weeks (10 vials): 3 bottles.

Alcohol Swabs:

• Per week: 14 swabs.
• 8 weeks: 112 (2 x 100-count). 16 weeks: 224 (3 x 100-count).

Important Notes

Practical considerations for consistency and safety.

• Use new sterile insulin syringes for each injection; dispose in a sharps container immediately after use.
• Rotate injection sites (abdomen, thighs, upper arms) systematically to reduce local irritation.
• Inject slowly and steadily; wait a few seconds before withdrawing the needle.
• Document daily dose, timing, and site rotation to maintain consistency throughout the protocol.
• Multi-use bacteriostatic water vials should be dated upon opening and discarded within 28 days.

How This Works

Selank is a synthetic analog of the immunomodulatory peptide tuftsin, consisting of the sequence Thr-Lys-Pro-Arg-Pro-Gly-Pro. In Russian clinical trials for anxiety disorders and neurasthenia, intranasal Selank produced anxiolytic effects comparable to benzodiazepines while also providing anti-asthenic (energy-boosting) benefits without sedative side effects or dependence potential. Preclinical studies in primates demonstrated that intranasal Selank eliminated fear and anxiety behaviors with long-term stabilizing effects on neurobehavior. Rodent studies confirmed robust anxiolytic efficacy comparable to classical tranquilizers. While most published human data utilized intranasal dosing, subcutaneous administration has shown effectiveness in research settings with consistent absorption and ease of dose measurement.

Benefits & Side Effects

Observations from clinical and preclinical literature.

• Produces anxiolytic effects comparable to benzodiazepines in human clinical trials without sedation or

dependence risk.

• Provides anti-asthenic (energy-boosting) benefits alongside anxiety reduction.
• Induces lasting neurobehavioral changes; primate studies showed elimination of fear/anxiety behaviors

with long-term stabilizing effects.

• Generally well tolerated in clinical studies; a 14-day continuous course was well-tolerated and effective in

human trials.

• Occasional mild injection-site reactions (redness, minor irritation) may occur with subcutaneous

administration.

• Lacks sedative side effects typical of classical anxiolytics, allowing for daytime use without impairment.

Lifestyle Factors

Complementary strategies for best outcomes.

• Maintain consistent sleep hygiene and aim for 7 to 9 hours of quality sleep to support neurological

function and stress resilience.

• Incorporate regular physical activity; both aerobic exercise and resistance training support mood

regulation and cognitive performance.

• Practice stress-management techniques such as mindfulness, meditation, or breathwork to complement

Selank's anxiolytic effects.

• Support nutritional status with adequate protein, omega-3 fatty acids, and micronutrients essential for

neurotransmitter synthesis.

• Minimize alcohol and stimulant use, which can interfere with anxiety management and neurological

stability.

Injection Technique

General subcutaneous guidance from clinical best-practice resources.

1. Clean the vial stopper and skin with alcohol; allow to dry.
2. Pinch a skinfold; insert the needle at 45 to 90 degrees into subcutaneous tissue.
3. Do not aspirate for subcutaneous injections; inject slowly and steadily.
4. Wait 5 to 10 seconds before withdrawing; dispose of syringe in sharps container.
5. Rotate sites systematically (abdomen, thighs, upper arms) to avoid lipohypertrophy.

References

Source citations for further reading.

1. Zozulia AA et al. The efficacy of Selank in highly anxious neurotic disorders (Russian clinical trial).
2. Selank in the treatment of generalized anxiety disorder and neurasthenia: clinical trial (Russian psychiatric literature).
3. Kolomin T et al. Selank-induced changes in gene expression in rat hippocampus (Neuroscience and Behavioral Physiology).
4. Anxiolytic effect of Selank in primates: experimental study (Russian primate neurobehavioral research).